Red Shoes are a trap item in the game. They are found on the 5th Floor of the house in Left (Mother's) Cell.
The red shoes can only be obtained after completing the White Pollen puzzle. After that the left cell door will unlock, allowing the player to obtain the red shoes hidden beneath a piece of cloth.
An unknown person will try to make you wear shoes. If you wear the red shoes, Viola's head will detach, and her body will go skipping away without her head before the 'Game Over' screen appears.
If the player has completed the Skull Puzzle, the waterway chamber will have water in it, which the tadpoles live in. The player has to wash the red shoes in the water in order to get the glass shoes. The glass shoes are used to walk over the poison in the Poison Hallway. After you have washed the shoes, the tadpoles will be dead.